V-type proton ATPase subunit F Antibody (1B8)

Images

 
Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 ...read more
Immunocytochemistry/ Immunofluorescence: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] Staining of COS7 cells transiently transfected by pCMV6-ENTRY V-type proton ATPase subunit F.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human thyroid tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Flow Cytometry: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-V-type ...read more
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-V-type proton ATPase subunit F mouse monoclonal ...read more
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal ...read more
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human Kidney tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human liver tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lung tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lymph node tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.
Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

V-type proton ATPase subunit F Antibody (1B8) Summary

Immunogen
Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP6V1F
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for V-type proton ATPase subunit F Antibody (1B8)

  • ATP6S14adenosinetriphosphatase 14k chain
  • ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
  • H(+)-transporting two-sector ATPase, 14kD subunit
  • MGC117321
  • MGC126037
  • MGC126038
  • vacuolar ATP synthase subunit F
  • vacuolar proton pump F subunit
  • Vacuolar proton pump subunit F
  • VATFATPase, vacuolar, 14 kD
  • V-ATPase 14 kDa subunit
  • V-ATPase F subunit
  • V-ATPase subunit F
  • Vma7
  • V-type proton ATPase subunit F

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ATP6V1F
Entrez