USP9x Antibody (2G7)

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Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human prostate tissue using anti-USP9x mouse monoclonal antibody.
Western Blot: USP9x Antibody (2G7) [NBP2-03824] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9x (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP9x mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP9x mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9x mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP9x mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human Kidney tissue using anti-USP9x mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human liver tissue using anti-USP9x mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clone
2G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

USP9x Antibody (2G7) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
USP9X
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
292 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-03824 in the following applications:

Publications
Read Publication using
NBP2-03824 in the following applications:

Reactivity Notes

Human, Dog, Rat, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for USP9x Antibody (2G7)

  • Deubiquitinating enzyme FAF-X
  • DFFRX
  • DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila)
  • Drosophila fat facets related, X-linked
  • EC 3.1.2.15
  • EC 3.4.19.12
  • FAF
  • FAM
  • Fat facets in mammals
  • fat facets protein related, X-linked
  • Fat facets protein-related, X-linked
  • hFAM
  • probable ubiquitin carboxyl-terminal hydrolase FAF-X
  • ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila)
  • ubiquitin specific peptidase 9, X-linked
  • ubiquitin thioesterase FAF-X
  • Ubiquitin thiolesterase FAF-X
  • ubiquitin-specific processing protease FAF-X
  • Ubiquitin-specific protease 9, X chromosome
  • Ubiquitin-specific-processing protease FAF-X
  • USP9
  • USP9x
  • X chromosome (fat facets-like Drosophila)

Background

This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP9x Antibody (NBP2-03824)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
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Review for USP9x Antibody (NBP2-03824) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using NBP2-03824:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 12/29/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24152793
SpeciesHuman
Special ApplicationsSee PMID 24152793

Product General Protocols

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Secondary Antibodies

 

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Anonymous
12/29/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol USP9X
Entrez
Uniprot