USP7 Antibody [DyLight 488]

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Fluorophore-linked immunosorbent assay: V5 Epitope Tag Antibody [DyLight 488] [NBP1-97344]

Product Details

Summary
Product Discontinued
View other related USP7 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-97344
    • Availability
      Product Discontinued

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USP7 Antibody [DyLight 488] Summary

Immunogen
Synthetic peptide: TDDPPRITQNPVC, corresponding to amino acids 30-41 of Human HAUSP.
Localization
Nuclear
Specificity
By Western blot, this detects a single clean band of about 130kD representing HAUSP in various cell lysates (see picture). The band is strongly depleted using peptide blocking with the immunising peptide.
Clonality
Polyclonal
Host
Rabbit
Gene
USP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:25000
  • Fluorophore-linked immunosorbent assay >1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Theoretical MW
128 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free Color: Green Excitation Wavelength: 493 Emission Wavelength: 518 Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester. Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for USP7 Antibody [DyLight 488]

  • Deubiquitinating enzyme 7
  • EC 3.1.2.15
  • EC 3.4.19.12
  • HAUSP
  • HAUSPTEF1
  • Herpes virus-associated ubiquitin-specific protease
  • Herpesvirus-associated ubiquitin-specific protease
  • TEF1
  • ubiquitin carboxyl-terminal hydrolase 7
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • ubiquitin specific protease 7 (herpes virus-associated)
  • Ubiquitin thioesterase 7
  • Ubiquitin-specific-processing protease 7
  • USP7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for V5 Epitope Tag Antibody (NBP1-97344)

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Bioinformatics

Gene Symbol USP7
Entrez