USP3 Antibody

Images

 
Western Blot: USP3 Antibody [NBP2-20831] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: USP3 Antibody [NBP2-20831] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.39 mg/ml

Order Details

USP3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human USP3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus MonkeyZebrafish (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.39 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP3 Antibody

  • Deubiquitinating enzyme 3
  • EC 3.1.2.15
  • EC 3.4.19.12
  • MGC129878
  • MGC129879
  • SIH003
  • ubiquitin carboxyl-terminal hydrolase 3
  • ubiquitin specific peptidase 3
  • ubiquitin specific protease 3
  • ubiquitin thioesterase 3
  • Ubiquitin thiolesterase 3
  • Ubiquitin-specific-processing protease 3
  • UBP

Background

Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol USP3
Uniprot