USP10 Antibody (2E1)

Images

 
Western Blot: USP10 Antibody (2E1) [NBP2-01452] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: USP10 Antibody (2E1) [NBP2-01452] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human prostate tissue using anti-USP10 mouse monoclonal antibody.
Flow Cytometry: USP10 Antibody (2E1) [NBP2-01452] - Analysis of Hela cells, using anti-USP10 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: USP10 Antibody (2E1) [NBP2-01452] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human breast tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human endometrium tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: USP10 Antibody (2E1) [NBP2-01452] - Staining of paraffin-embedded Human pancreas tissue using anti-USP10 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

USP10 Antibody (2E1) Summary

Immunogen
Full length human recombinant protein of human USP10(NP_005144) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
USP10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Dog, Rat, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for USP10 Antibody (2E1)

  • Deubiquitinating enzyme 10
  • EC 3.4.19.12
  • KIAA0190MGC2621
  • ubiquitin carboxyl-terminal hydrolase 10
  • ubiquitin specific peptidase 10
  • ubiquitin specific protease 10
  • ubiquitin thioesterase 10
  • Ubiquitin Thiolesterase 10
  • Ubiquitin-specific-processing protease 10
  • UBPO
  • Uchrp
  • USP10

Background

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol USP10
Entrez