URI Overexpression Lysate (Native)

Images

 
Western Blot: URI Overexpression Lysate (Adult Normal) [NBL1-08262] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for URI.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

URI Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209452 and based on accession number NM_134447. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 19 open reading frame 2 (C19orf2), transcript variant 2, mRNA.
Gene
URI1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for URI Overexpression Lysate (Native)

  • chromosome 19 open reading frame 2
  • FLJ10575
  • NNX3RPB5-mediating protein
  • Protein NNX3
  • RMPunconventional prefoldin RPB5 interactor
  • RNA polymerase II subunit 5-mediating protein
  • URIRNA polymerase II, subunit 5-mediating protein

Background

The protein encoded by this gene binds to RNA polymerase II subunit 5 (RPB5) and negatively modulates transcription through its binding to RPB5. The encoded protein seems to have inhibitory effects on various types of activated transcription, but it requires the RPB5-binding region. This protein acts as a corepressor. It is suggested that it may require signaling processes for its function or that it negatively modulates genes in the chromatin structure. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Transcript Variant: This variant (2) lacks 35 nt in the coding region, resulting in a frameshift at the 5' end, compared to variant 1. It encodes an isoform b that is shorter and has a different N-terminus than isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol URI1