UQCRC1 Antibody (139) - Azide and BSA Free

Western Blot: UQCRC1 Antibody (139) [NBP2-43597] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139].

Immunocytochemistry/Immunofluorescence: UQCRC1 Antibody (139) [NBP2-43597] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: UQCRC1 protein stained by UQCRC1 antibody [139] diluted at 1:100. Blue: Hoechst 33342 staining.

Western Blot: UQCRC1 Antibody (139) [NBP2-43597] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE UQCRC1 antibody [139] dilution: 1:1000

Western Blot: UQCRC1 Antibody (139) [NBP2-43597] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE UQCRC1 antibody [139] dilution: 1:1000

Western Blot: UQCRC1 Antibody (139) [NBP2-43597] - Analysis of A. 50 ug rat brain lysate/extract 10 % SDS-PAGE UQCRC1 antibody [139] dilution: 1:1000

• Reactivity: Hu, Mu, Rt, Pm, RM
• Applications: WB, ICC/IF
• Clone: 139
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

UQCRC1 Antibody (139) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (97%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: UQCRC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for UQCRC1 Antibody (139) - Azide and BSA Free

• Complex III subunit 1
• Core protein I
• cytochrome b-c1 complex subunit 1, mitochondrial
• D3S3191
• QCR1
• ubiquinol-cytochrome c reductase core protein I
• Ubiquinol-cytochrome-c reductase complex core protein 1
• UQCR1

Background

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UQCRC1