Primate Universal RNA Tissue Lysate (Adult Normal) Summary
Monkey Normal Tissues
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Product contains 100ug in each vial.
Lysate Details for Universal RNA
Quality Control 1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-HCl, pH 7.5) 2. The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100 bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number).7, and the area of 28s over 18s ratio.1. 3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR 4. cDNA synthesis is successfully performed by using this RNA as template 5. Universal RNA is labeled and hybridized to a cDNA microarray, expected result of gene expression is consistent with each lot.
Monkey Universal RNA is prepared from major organs of both male and female Cynomolgus and Rhesus monkey adult donors. This total RNA serves as a standard for comparison of gene expression from microarray studies
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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