Universal RNA Lysate

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-71699
    • Availability
      Product Discontinued

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Universal RNA Lysate Summary

Specificity
Monkey Normal Tissues

Applications/Dilutions

Application Notes
Product contains 100ug in each vial.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Concentration
Please contact technical services for concentration.

Lysate Details for Universal RNA

Life Stage
Adult
Tissue
Universal
Tissue Condition
Normal

Notes

Quality Control 1. The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-HCl, pH 7.5) 2. The Universal RNA is analyzed by capillary electrophoresis with Agilent 2100 bioanalyzer. RNA integrity is determined with RIN (RNA Integrity Number).7, and the area of 28s over 18s ratio.1. 3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR 4. cDNA synthesis is successfully performed by using this RNA as template 5. Universal RNA is labeled and hybridized to a cDNA microarray, expected result of gene expression is consistent with each lot.

Background

Monkey Universal RNA is prepared from major organs of both male and female Cynomolgus and Rhesus monkey adult donors. This total RNA serves as a standard for comparison of gene expression from microarray studies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Calculators

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