UNG Antibody (k1C12)

Images

 
Western Blot: UNG Antibody (k1C12) [NBP1-04352] - Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG (1:1000). Proteins were visualized using a goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
k1C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

UNG Antibody (k1C12) Summary

Immunogen
Recombinant human UNG(1-313aa) purified from E. coli
Specificity
Anti-human UNG mAb, clone k1C12, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UNG protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UNG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for UNG Antibody (k1C12)

  • DGU
  • DGUEC 3.2.2
  • HIGM4EC 3.2.2.27
  • UDG
  • UDGDKFZp781L1143
  • UNG
  • UNG15
  • UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
  • UNG2
  • uracil-DNA glycosylase

Background

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UNG
Entrez
Uniprot