UCP3 Antibody

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Western Blot: UCP3 Antibody [NBP2-20797] - A. 30 ug RD whole cell lysate/extract 12 % SDS-PAGE UCP3 antibody dilution: 1:1000
Western Blot: UCP3 Antibody [NBP2-20797] - A. 50 ug rat muscle lysate/extract 12 % SDS-PAGE UCP3 antibody dilution: 1:500

Product Details

Summary
Reactivity Hu, Rt, Po, CaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.66 mg/ml

Order Details

UCP3 Antibody Summary

Immunogen
Full length human UCP3 Recombinant protein.
Localization
Mitochondrion inner membrane; Multi-pass membrane protein
Predicted Species
Porcine (90%), Canine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCP3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (85%), Sheep (87%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.66 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCP3 Antibody

  • mitochondrial uncoupling protein 3
  • SLC25A9Solute carrier family 25 member 9
  • UCP 3
  • uncoupling protein 3 (mitochondrial, proton carrier)
  • Uncoupling protein-3

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UCP3
Uniprot