UCP2 Antibody

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Immunohistochemistry-Paraffin: UCP2 Antibody [NBP1-51221] Immunohistochemistry (IHC) analyzes of UCP2 (H113) pAb in paraffin-embedded human lung carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51221
    • Availability
      Product Discontinued

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UCP2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human UCP2.
Specificity
UCP2 (H113) pAb detects endogenous levels of UCP2 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
UCP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Immunohistochemistry (IHC) analyzes of UCP2 (H113) pAb in paraffin-embedded human lung carcinoma tissue.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCP2 Antibody

  • mitochondrial uncoupling protein 2
  • SLC25A8
  • SLC25A8BMIQ4
  • Solute carrier family 25 member 8
  • UCP 2
  • UCP2
  • UCPH
  • uncoupling protein 2 (mitochondrial, proton carrier)

Background

UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for UCP2 Antibody (NBP1-51221). (Showing 1 - 1 of 1 FAQ).

  1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
    • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol UCP2
Entrez