UCH-L3 Antibody

Images

 
Western Blot: UCH-L3 Antibody [NBP1-55316] - MCF7 cell lysate, Antibody Titration: 0.2-1 ug/ml.
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316] - Human placenta tissue at an antibody concentration of 4-8ug/ml.
Western Blot: UCH-L3 Antibody [NBP1-55316] - Antibody Positive control: Lane 1 : 30ug Zebrafish skin lysate Lane 2: 30ug Zebrafish liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit-HRP ...read more

Product Details

Summary
Reactivity Hu, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

UCH-L3 Antibody Summary

Immunogen
Synthetic peptides corresponding to UCHL3(ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)) The peptide sequence was selected from the N terminal of UCHL3. Peptide sequence MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UCHL3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
Application Notes
This is a rabbit polyclonal antibody against UCHL3 and was validated on Western blot.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for UCH-L3 Antibody

  • EC 3.4.19.12
  • ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L3
  • Ubiquitin Thioesterase L3
  • ubiquitin thiolesterase
  • UCHL3
  • UCH-L3

Background

UCHL3 is an ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol UCHL3
Entrez
Uniprot