UCH-L1/PGP9.5 Antibody

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Western Blot: PGP9.5 / UCHL-1 Antibody [NBP1-49850] - Western blot analysis of PGP9.5 / UCHL-1 (aa 58-68) in Human Hippocampus lysate (35ug protein in RIPA buffer) using NBP1-49850 at 0.01ug/ml. Primary incubation was 1 ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-49850
    • Availability
      Product Discontinued

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UCH-L1/PGP9.5 Antibody Summary

Immunogen
Peptide with sequence C-QHENFRKKQIE corresponding to internal region according to NP_004172.2.
Epitope
QHENFRKKQIE - internal region
Marker
pan-Neuronal Marker
Clonality
Polyclonal
Host
Goat
Gene
UCHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
EIA is reported to work in Sandwich ELISA both as a capturer and as a reporter in combination with NBP1-49851. WB: Approx. 26 kDa band observed in human brain (cerebellum and hippocampus) and mouse and rat brain lysates (calculated MW of 24.8 kDa band according to human NP_004172.2, mouse NP_035800.2 and rat NP_058933.2).
Publications
Read Publication using NBP1-49850.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCH-L1/PGP9.5 Antibody

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

Protein gene product 9.5 (PGP9.5) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of PGP9.5 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCH-L1/PGP9.5 Antibody (NBP1-49850)(1)

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Bioinformatics

Gene Symbol UCHL1
Entrez
Uniprot