UCH-L1/PGP9.5 Antibody (EPR4118)

Product Discontinued

UCH-L1/PGP9.5 Antibody (EPR4118) Summary

• Immunogen: A synthetic peptide corresponding to residues in human PGP9.5 was used as an immunogen.
• Marker: pan-Neuronal Marker
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UCHL1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:10-100
  • Western Blot 1:1000-10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:33728539).
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for UCH-L1/PGP9.5 Antibody (EPR4118)

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

PGP9.5 belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. It is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in PGP9.5 may be associated with Parkinson disease and Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UCH-L1/PGP9.5 Antibody (NBP1-96612)(3)

Publications using NBP1-96612

• Russo C, Mannino G, PatanE M et al. Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells Histochemistry and cell biology 2021-03-16 [PMID: 33728539] (ICC/IF, FLOW, Human)
• Corsetti V, Florenzano F, Atlante A et al. NH2-truncated human tau induces deregulated mitophagy in neurons by aberrant recruitment of Parkin and UCHL-1: implications in Alzheimer's Disease Hum. Mol. Genet. 2015-02-15 [PMID: 25687137] (IP, Human)
• Y-A Liu, Y Chen et al. Optical clearing improves the imaging depth and signal-to-noise ratio for digital analysis and three-dimensional projection of the human enteric nervous system ,Neurogastroenterol Motil. [PMID: 21895876] (IF/IHC)

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
  • 22223(Mouse)
  • 29545(Rat)
• Uniprot:
  • P09936(Human)