UCH-L1/PGP9.5 Antibody (2B5)

Images

 
Western Blot: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - Analysis of extracts (35ug) from 9 different cell lines by using anti-UCHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: ...read more
Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - Staining of paraffin-embedded Human Kidney tissue using anti-UCHL1 mouse monoclonal antibody.
Flow Cytometry: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UCHL1 antibody, and then ...read more
Western Blot: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UCHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UCHL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody (2B5) [NBP2-01769] - Staining of paraffin-embedded Human breast tissue using anti-UCHL1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
2B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

UCH-L1/PGP9.5 Antibody (2B5) Summary

Immunogen
Full length human recombinant protein of human UCHL1(NP_004172) produced in HEK293T cell.
Marker
pan-Neuronal Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Rat, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for UCH-L1/PGP9.5 Antibody (2B5)

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UCHL1
Entrez