Recombinant Human Ubiquitin His Protein

Images

 
Western Blot: Ubiquitin Protein [NBP1-42465] - The anti-Ubiquitin antibody NB300-129 was used to detect this protein on a western blot. 500ng of protein were loaded on the gel and then detected with NB300-129.
SDS-Page: Ubiquitin Protein [NBP1-42465] - This RPS27a Human Recombinant protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded. The purity of the protein is >95%.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Ubiquitin His Protein Summary

Description
A full-length recombinant protein with an N-terminal His tag and corresponding to amino acids 1-156 of Ubiquitin. Accession #: P62979.

Source: E. coli

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RPS27A
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
This protein is useful as a positive control in Western Blotting and SDS-PAGE.
Theoretical MW
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
28 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
Preservative
N/A
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Ubiquitin His Protein

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. This protein is encoded by the gene RPS27A, which also encodes for an N-terminal ubiquitin fusion partner. It contains C4-type zinc finger domains and is located in the cytoplasm. Human RPS27A with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol RPS27A
Entrez