Recombinant Human UbcH3/Cdc34 Protein

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SDS-Page: UbcH3/Cdc34 Recombinant Protein [NBP1-48388]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human UbcH3/Cdc34 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-236 of Human UbcH3/Cdc34

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CDC34
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human UbcH3/Cdc34 Protein

  • Cdc34
  • cell division cycle 34 homolog (S. cerevisiae)
  • cell division cycle 34
  • E2-CDC34
  • EC 6.3.2.19
  • UBC3
  • UbcH3
  • UBE2R1
  • UBE2R1UBCH3
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 R1
  • Ubiquitin-conjugating enzyme E2-32 kDa complementing
  • Ubiquitin-conjugating enzyme E2-CDC34
  • Ubiquitin-protein ligase R1

Background

CDC34 is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. CDC34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae CDC34, which is essential for the G1 to S phase transition. Recombinant human CDC34 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CDC34
Entrez