UbcH3/Cdc34 Overexpression Lysate (Native)

Images

 
Western Blot: Cdc34 Overexpression Lysate (Adult Normal) [NBL1-09001] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdc34.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

UbcH3/Cdc34 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203517 and based on accession number NM_004359. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cell division cycle 34 homolog (S. cerevisiae) (CDC34), mRNA.
Gene
CDC34

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for UbcH3/Cdc34

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for UbcH3/Cdc34 Overexpression Lysate (Native)

  • Cdc34
  • cell division cycle 34 homolog (S. cerevisiae)
  • cell division cycle 34
  • E2-CDC34
  • EC 6.3.2.19
  • UBC3
  • UbcH3
  • UBE2R1
  • UBE2R1UBCH3
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 R1
  • Ubiquitin-conjugating enzyme E2-32 kDa complementing
  • Ubiquitin-conjugating enzyme E2-CDC34
  • Ubiquitin-protein ligase R1

Background

The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for UbcH3/Cdc34 Lysate (NBL1-09001) (0)

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Bioinformatics

Gene Symbol CDC34
Entrez