UbcH3/Cdc34 Antibody

Images

 
Western Blot: UbcH3/Cdc34 Antibody [NBP1-32153] - Sample (30ug whole cell lysate) A:Raji 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: ...read more
Immunocytochemistry/ Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.61 mg/ml

Order Details

UbcH3/Cdc34 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CDC34. The exact sequence is proprietary.
Predicted Species
Rat (98%), Xenopus (90%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC34
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.61 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UbcH3/Cdc34 Antibody

  • Cdc34
  • cell division cycle 34 homolog (S. cerevisiae)
  • cell division cycle 34
  • E2-CDC34
  • EC 6.3.2.19
  • UBC3
  • UbcH3
  • UBE2R1
  • UBE2R1UBCH3
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 R1
  • Ubiquitin-conjugating enzyme E2-32 kDa complementing
  • Ubiquitin-conjugating enzyme E2-CDC34
  • Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC34
Entrez