UbcH3/Cdc34 Antibody (14B5)

Images

 
Western Blot: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Analysis of paraformaldehyde-fixed HeLa, using CDC34 antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: UbcH3/Cdc34 Antibody (14B5) [NB500-168] - Analysis of paraffin-embedded HBL435 xenograft, using CDC34 antibody at 1:200 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
14B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

UbcH3/Cdc34 Antibody (14B5) Summary

Immunogen
Full-length human Cdc34 expressed in E. coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDC34
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Positive Control
293 Lysate (NB800-PC6)
UbcH3/Cdc34 Lysate (NBL1-09001)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for UbcH3/Cdc34 Antibody (14B5)

  • Cdc34
  • cell division cycle 34 homolog (S. cerevisiae)
  • cell division cycle 34
  • E2-CDC34
  • EC 6.3.2.19
  • UBC3
  • UbcH3
  • UBE2R1
  • UBE2R1UBCH3
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2 R1
  • Ubiquitin-conjugating enzyme E2-32 kDa complementing
  • Ubiquitin-conjugating enzyme E2-CDC34
  • Ubiquitin-protein ligase R1

Background

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UbcH3/Cdc34 Antibody (NB500-168) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-168AF405
Alexa Fluor 488 Labeled NB500-168AF488
Alexa Fluor 647 Labeled NB500-168AF647
Alexa Fluor 700 Labeled NB500-168AF700
DyLight 350 Labeled NB500-168UV
DyLight 405 Labeled NB500-168V
DyLight 488 Labeled NB500-168G
DyLight 680 Labeled NB500-168FR
DyLight 755 Labeled NB500-168IR

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Bioinformatics

Gene Symbol CDC34
Entrez