u-Plasminogen Activator/Urokinase Antibody (PGM2005) Summary
The antibody reacts with the Urokinase antigen.
The antibody binds to human high molecular weight single-chain urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high molecular weight two-chain urokinase type plasminogen activator (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high affinity (Kd= 0.1 nM) and does not inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not inhibited. The antibody is highly specific for urokinase, no cross reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase. cross reacts with rat scu/tcu PA.
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- Western Blot 1:100-1:2000
- ELISA 1:100-1:2000
The antibody can be used for targeting of urokinase to trombi, cells or extracellular matrix in vitro and in vivo. The antibody can also be used for quantitative, specific and sensitive assay for urokinase in human fluids (RIA, ELISA) in combination withThe antibody is very stable and can, after appropriate binding, be used over 20-40 times in immunopurification of urokinase. This antibody can also be used for immunohistochemical studies with a dilution of 1:30 -1:50. For ELISA start diluting at 1: 5.0
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Alternate Names for u-Plasminogen Activator/Urokinase Antibody (PGM2005)
- EC 3.4.21
- EC 126.96.36.199
- plasminogen activator, urokinase
- uPlasminogen Activator
- u-Plasminogen Activator
- urokinase-type plasminogen activator
This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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