U-2 OS Whole Cell Lysate

Product Discontinued

U-2 OS Whole Cell Lysate Summary

• Localization: Bone
• Specificity: U-2 OS (human osterogenic sarcoma) whole cell lysate (non-denatured)

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Concentration: 5 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Bone/Cartilage
• Protein State: Native
• Subcellular Fraction: Whole

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

U2OS is a human bone osteosarcoma from a human 15 year old Caucasian female expressing wild type p53 and lacking p16. U2OS also has a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus. U2OS cells were established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.