U-2 OS Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
3 mg/ml

Order Details

U-2 OS Whole Cell Lysate Summary

Description
Whole cell lysate (denatured)
Localization
Bone
Preparation
Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay. The lysate was mixed in 5X Sample Buffer to become final Sample Buffer as Storage Buffer. The lysate was heated at 95C for 5 min and cooled rapidly.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
In ready-to-use Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% Glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue). Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
Concentration
3 mg/ml

Lysate Details for U-2 OS

Type
Cell
Tissue
Bone/Cartilage
Protein State
Denatured
Subcellular Fraction
Whole

Notes

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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U-2 OS L022V1

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