TWA1 Antibody

Western Blot: TWA1 Antibody [NBP1-32596] - ARMC8 HEK293 CRISPR knockout cells were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-46279-5), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TWA1 Antibody [NBP1-32596] - Paraformaldehyde-fixed HeLa, using antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: TWA1 Antibody [NBP1-32596] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Western Blot: TWA1 Antibody [NBP1-32596] - (30 ug whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000

Knockout Validated: TWA1 Antibody [NBP1-32596] - TWA1 HEK293 CRISPR knockout cells were analyzed by Western blot with antibodies to CTLH complex members, as indicated. Vinculin was used as a loading control. Quantifications are shown below each blot and protein levels are shown relative to control cells set to 1 and normalized to Vinculin levels. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-46279-5), licensed under a CC-BY license.

• Reactivity: Hu, Rt, Mu, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TWA1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human C20orf11. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Rhesus Macaque (100%), Bovine (97%), Xenopus (96%), Chicken (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GID8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: IHC-Fr (Frozen sections) Assay dependent
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for TWA1 Antibody

• bA305P22.1
• C20orf11
• chromosome 20 open reading frame 11
• FLJ20602
• GID complex subunit 8 homolog (S. cerevisiae)
• Twa1
• TWA1protein C20orf11
• two hybrid associated protein 1
• Two hybrid-associated protein 1 with RanBPM

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TWA1 Antibody (NBP1-32596)(3)

Publications using NBP1-32596

• Onea G, Maitland MER, Wang X et al. Distinct nuclear and cytoplasmic assemblies and interactomes of the mammalian CTLH E3 ligase complex Journal of cell science 2022-07-15 [PMID: 35833506]
• Sherpa D, Chrustowicz J, Qiao S et al. GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme Molecular cell 2021-04-22 [PMID: 33905682] (WB, Human)
• Maitland MER, Onea G, Chiasson CA et al. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation Sci Rep 2019-07-08 [PMID: 31285494] (WB, Human)

Bioinformatics

• Gene Symbol: GID8