Tubulin Antibody (YOL1/34)

Images

 
Western Blot: Tubulin Antibody (YOL1/34) [NB100-1639] - Western for tubulin in Jurkat and THP1 cells. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (YOL1/34) [NB100-1639] - HeLa cells.
Western Blot: Tubulin Antibody (YOL1/34) [NB100-1639] - WB analysis of Tubulin and CD107b proteins on lysate of J774 cells (mouse BALB/c monocyte macrophages cell line) using different dilutions of rat anti tubulin ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Dr, Fu, YeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, RIA
Clone
YOL1/34
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Tubulin Antibody (YOL1/34) Summary

Immunogen
Yeast tubulin
Marker
Microtubule Marker
Specificity
This detects an epitope on alpha-tubulin between the amino acids 414-422. It has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Radioimmunoassay 1:100-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NB100-1639 in the following applications:

Publications
Read Publications using
NB100-1639 in the following applications:

  • 5 publications
  • WB
    6 publications

Reactivity Notes

Cross reacts with Arabidopsis, Saccharomyces, Platyzoma, Ashbya, and Naegleria. Based on sequence similarity, is expected to react with: Birds and Mammals.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Tubulin Antibody (YOL1/34)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
AF7068
Species: Hu
Applications: WB, IHC, ICC
NB120-22104
Species: Hu, Mu, Rt
Applications: WB
NBP2-46250
Species: Hu, Mu, Ca, Mk
Applications: WB, IHC, IF
NB600-1081
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
MAB1195
Species: Multi
Applications: WB, Simple Western, ICC
NBP1-78162
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-02474
Species: Hu, Rt, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
NB600-936
Species: Hu, Mu, Rt, Po, Bv, Ch, ChHa, In, Pm, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-43837
Species: Hu, Mu, Rt, Dr, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-31528
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-86999
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31004
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88607
Species: Hu
Applications: WB, IHC, IHC-P
H00002316-M01
Species: Hu
Applications: WB, ELISA, IHC-P, PLA
NB100-1639
Species: Hu, Mu, Rt, Dr, Fu, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, RIA

Publications for Tubulin Antibody (NB100-1639)(12)

We have publications tested in 4 confirmed species: Human, Drosophila, Fungi, Yeast.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(5)
WB
(6)
All Applications
Filter By Species
Human
(2)
Drosophila
(1)
Fungi
(2)
Yeast
(6)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-1639 Applications Species
Huang X, Chen X, He Y et al. Mitochondrial complex I bridges a connection between regulation of carbon flexibility and gastrointestinal commensalism in the human fungal pathogen Candida albicans. PLoS Pathog. 2017 Jun 01 [PMID: 28570675] (WB, Human) WB Human
Newnham L, Jordan PW, Carballo JA et al. Ipl1/Aurora Kinase Suppresses S-CDK-Driven Spindle Formation during Prophase I to Ensure Chromosome Integrity during Meiosis. PLoS One 2013 Dec 27 [PMID: 24386320] (ICC/IF, Yeast) ICC/IF Yeast
Whitfield ZJ, Chisholm J, Hawley RS, Orr-Weaver TL. A Meiosis-Specific Form of the APC/C Promotes the Oocyte-to-Embryo Transition by Decreasing Levels of the Polo Kinase Inhibitor Matrimony. PLoS Biol. 2013 Sep [PMID: 24019759] (WB, Drosophila) WB Drosophila
Pande K, Chen C, Noble SM et al. Passage through the mammalian gut triggers a phenotypic switch that promotes Candida albicans commensalism. Nat Genet 2013 Jul 28 [PMID: 23892606] (WB, Fungi) WB Fungi
Ding X, Yu Q, Xu N et al. Ecm7, a regulator of HACS, functions in calcium homeostasis maintenance, oxidative stress response and hyphal development in Candida albicans. Fungal Genet Biol 2013 Jun 13 [PMID: 23769872] (WB, Yeast) WB Yeast
Chen C, Noble SM. Post-Transcriptional Regulation of the Sef1 Transcription Factor Controls the Virulence of Candida albicans in Its Mammalian Host PLoS Pathog 2012 Nov [PMID: 23133381] (WB, Fungi) WB Fungi
Hwang WW, Madhani HD. Nonredundant requirement for multiple histone modifications for the early anaphase release of the mitotic exit regulator Cdc14 from nucleolar chromatin. PLoS Genet. 2009 Aug [PMID: 19662160] (ICC/IF, Yeast) ICC/IF Yeast
Yu L, Lopez A, Anaflous A et al. Chemical-genetic profiling of imidazo[1,2-a]pyridines and -pyrimidines reveals target pathways conserved between yeast and human cells. PLoS Genet. 2008 Nov [PMID: 19043571] (WB, Yeast) WB Yeast
Yamamoto A, Nunoshiba T, Umezu K et al. Phenyl hydroquinone, an Ames test-negative carcinogen, induces Hog1-dependent stress response signaling. FEBS J. 2008 Nov [PMID: 18959758] (ICC/IF, Yeast) ICC/IF Yeast
Venkatesan, RN et al. Mutation at the Polymerase Active Site of Mouse DNA Polymerase delta Increases Genomic Instability Accelerates Tumorigenesis. Mol Cell Biol;27:7669-7682. Nov-07. [PMID: 17785453]
Show All 12 Publications.

Review for Tubulin Antibody (NB100-1639) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-1639:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Tubulin NB100-1639
Enlarge
reviewed by:
Anonymous
WB Human 09/26/2014
View

Summary

ApplicationWestern Blot
Sample TestedJurkat and THP1 cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionDonkey anti-Rat IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB73266
Secondary ConcentrationDiluted 5,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 10 sec exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB100-1639). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1639AF405
Alexa Fluor 488 Labeled NB100-1639AF488
Alexa Fluor 647 Labeled NB100-1639AF647
Alexa Fluor 700 Labeled NB100-1639AF700
DyLight 350 Labeled NB100-1639UV
DyLight 405 Labeled NB100-1639V
DyLight 488 Labeled NB100-1639G
DyLight 680 Labeled NB100-1639FR
DyLight 755 Labeled NB100-1639IR

Additional Tubulin Products

Tubulin NB100-1639

Bioinformatics Tool for Tubulin Antibody (NB100-1639)

Discover related pathways, diseases and genes to Tubulin Antibody (NB100-1639). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Tubulin Antibody (NB100-1639)

Discover more about diseases related to Tubulin Antibody (NB100-1639).
 

Pathways for Tubulin Antibody (NB100-1639)

View related products by pathway.

PTMs for Tubulin Antibody (NB100-1639)

Learn more about PTMs related to Tubulin Antibody (NB100-1639).
 

Research Areas for Tubulin Antibody (NB100-1639)

Find related products by research area.

Blogs on Tubulin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/26/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TUBA1A
Entrez
Uniprot