Tubulin Antibody (YL1/2) [PerCP]

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Product Details

Summary
Product Discontinued
View other related Tubulin Primary Antibodies

Order Details


    • Catalog Number
      NB600-506PCP
    • Availability
      Product Discontinued

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Tubulin Antibody (YL1/2) [PerCP] Summary

Immunogen
Full length native protein (purified) (S. cerevisiae).
Epitope
A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
Marker
Microtubule Marker
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TUBA1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Radioimmunoassay
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.

Reactivity Notes

Human, mouse, rat, monkey, S. cerevisiae, S. pombe, Slime moulds, Allium and birds. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759) Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Tubulin Antibody (YL1/2) [PerCP]

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB600-506PCP). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB600-506APC
DyLight 350 Labeled NB600-506UV
DyLight 405 Labeled NB600-506V
DyLight 488 Labeled NB600-506G
DyLight 680 Labeled NB600-506FR
DyLight 755 Labeled NB600-506IR
FITC Labeled NB600-506F
PE Labeled NB600-506PE

Additional Tubulin Products

Tubulin NB600-506PCP

Bioinformatics Tool for Tubulin Antibody (NB600-506PCP)

Discover related pathways, diseases and genes to Tubulin Antibody (NB600-506PCP). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for Tubulin Antibody (NB600-506PCP)

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Research Areas for Tubulin Antibody (NB600-506PCP)

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Blogs on Tubulin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA1A