Tubulin Antibody (TU-01)

Images

 
Western Blot: Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (TU-01) [NB500-333] - Tubulin filament in HeLa. Image from verified customer review.
Western Blot: Tubulin Antibody (TU-01) [NB500-333] - Fig. 4. Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of lysates of various cell ...read more
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (TU-01) [NB500-333] - Fig. 1. Saining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus ...read more
Immunocytochemistry/ Immunofluorescence: Tubulin Antibody (TU-01) [NB500-333] - Fig. 2.Staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).
Immunoprecipitation: Tubulin Antibody (TU-01) [NB500-333] - alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ma, PlSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
TU-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Tubulin Antibody (TU-01) Summary

Immunogen
Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.
Localization
Major constituent of microtubules.
Marker
Microtubule Marker
Specificity
This recognizes the defined epitope (aa 65-97) on N-terminal structureal domain of alpha tubulin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
In WB incubation 60 min in room temperature. Positive control: HPB-ALL human peripheral blood leukemia cell line (incubation 60 min) Porcine brain lysate (incubation 90 min) Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. IHC-P Positive tissue: heart ICC: Staining technique: fixed and permeablized cells. Purity is > 95% (by SDS-PAGE).
Reviewed Applications
Read 1 Review rated 5
using
NB500-333 in the following applications:

Publications
Read Publication using
NB500-333 in the following applications:

  • WB
    1 publication

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26594217).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Tubulin Antibody (TU-01)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB500-333
Species: Hu, Mu, Rt, Ma, Pl
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for Tubulin Antibody (NB500-333)(1)

We have publications tested in 1 confirmed species: Plant.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Tubulin Antibody (NB500-333) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB500-333:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Tubulin NB500-333
Enlarge
reviewed by:
Anonymous
WB Human 03/15/2016
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Summary

ApplicationWestern Blot
Sample TestedHEK293T and NIH3T3 whole cell lysate
SpeciesHuman
Lot518390

Blocking

Blocking Details2% BSA in PBS for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:3000 in 2% BSA-PBS overnight at 4C

Secondary Antibody

Secondary Descriptionanti-mouse HRP
Secondary Manufacturer Cat#Novusbio cat# NB7539
Secondary Concentration1:5000

Details

Detection Notesdetection with ECL, exposure time is 10 seconds

Comments

CommentsThis mouse anti-tubulin antibody is super clean. The bands are very strong with just 5 to 10 seconds exposure time. It is a very good loading control antibody for western blot.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NB500-333). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Additional Tubulin Products

Tubulin NB500-333

Bioinformatics Tool for Tubulin Antibody (NB500-333)

Discover related pathways, diseases and genes to Tubulin Antibody (NB500-333). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Tubulin Antibody (NB500-333)

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Pathways for Tubulin Antibody (NB500-333)

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Research Areas for Tubulin Antibody (NB500-333)

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Blogs on Tubulin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
03/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol TUBA1A
Entrez
Uniprot