Tubulin Antibody (10D8)


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Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB
0.5 mg/ml

Order Details

Tubulin Antibody (10D8) Summary

This antibody was generated by immunizing host with Recombinant full length human α-tubulin
Microtubule Marker
This antibody has been tested to recognize phosphorylated, nonphosphorylated, acetylated and nonacetylated forms.
IgM Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Phosphate-buffered solution, pH 7.2.
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescent staining applications, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Read Publication using
NBP1-48667 in the following applications:

  • WB
    1 publication

Reactivity Notes

This protein is highly conserved in most species. We have validate cross reaction in hamun and mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for Tubulin Antibody (10D8)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific


Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting. RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tubulin Antibody (NBP1-48667)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

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Showing Publication 1 - 1 of 1.
Publication using NBP1-48667 Applications Species
Zhao M, Gjerset RA. Topoisomerase-I PS506 as a Dual Function Cancer Biomarker. PLoS ONE. 2015 Aug 07 [PMID:26248194] (WB, Human) WB Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Tubulin Antibody (NBP1-48667). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies


Isotype Controls

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Tubulin NBP1-48667

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Blogs on Tubulin.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol TUBA1A

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