Tubulin Antibody (10D8)

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Tubulin Antibody (10D8) [NBP1-48667] - Total cell lysates (15 ug protein) from HaCaT, HeLa, and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with Biotin ...read more

Product Details

Summary
Product Discontinued
View other related Tubulin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-48667
    • Availability
      Product Discontinued

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Tubulin Antibody (10D8) Summary

Immunogen
Recombinant full length human alpha-tubulin
Localization
Ubiquitous
Marker
Microtubule Marker
Specificity
This antibody has been tested to recognize phosphorylated, nonphosphorylated, acetylated and nonacetylated forms.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TUBA1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
Application Notes
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescent staining applications, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Publications
Read Publication using
NBP1-48667 in the following applications:

  • WB
    1 publication

Reactivity Notes

This protein is highly conserved in most species. We have validate cross reaction in human and mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Tubulin Antibody (10D8)

  • Alpha-tubulin 3
  • B-ALPHA-1
  • FLJ25113
  • hum-a-tub2
  • LIS3
  • TUBA3hum-a-tub1
  • tubulin alpha-1A chain
  • Tubulin alpha-3 chain
  • Tubulin B-alpha-1
  • tubulin, alpha 1a
  • tubulin, alpha 3
  • tubulin, alpha, brain-specific

Background

Tubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes alpha-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting. RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tubulin Antibody (NBP1-48667)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tubulin Antibody (NBP1-48667). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in buying a Tubulin antibody to use in Western blot as a loading control. We would like to use it on rat heart samples. Can you make a recommendation?
    • I would recommend the Tubulin Antibody (YL1/2) catalog # NB600-506. This antibody works great as a loading control in Western blot and has been validated for use with rat samples.

Secondary Antibodies

 

Isotype Controls

Additional Tubulin Products

Tubulin NBP1-48667

Bioinformatics Tool for Tubulin Antibody (NBP1-48667)

Discover related pathways, diseases and genes to Tubulin Antibody (NBP1-48667). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Tubulin Antibody (NBP1-48667)

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Pathways for Tubulin Antibody (NBP1-48667)

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PTMs for Tubulin Antibody (NBP1-48667)

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Research Areas for Tubulin Antibody (NBP1-48667)

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Blogs on Tubulin.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

Who Needs Antibodies for Tubulin Modifications?
Tubulin is a heterodimeric complex composed of alpha and beta subunits. Just like histones, tubulin can undergo post-translational modification, to affect cellular function. Novus Biologicals offers a large database of modified Tubulin antibody produc...  Read full blog post.

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Bioinformatics

Gene Symbol TUBA1A