Tubulin alpha-8 Antibody (2G6)

Images

 
Western Blot: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] WB analysis of extracts (35ug) from 9 different cell lines by using anti-Tubulin alpha-8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-8.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of paraffin-embedded prostate tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Western Blot: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-8 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of paraffin-embedded Kidney tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Tubulin alpha-8 Antibody (2G6) [NBP2-02474] - Staining of paraffin-embedded pancreas tissue using anti-Tubulin alpha-8 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Tubulin alpha-8 Antibody (2G6) Summary

Immunogen
Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBA8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Dog, Rat, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Tubulin alpha-8 Antibody (2G6)

  • Alpha-tubulin 8
  • TUBAL2Tubulin alpha chain-like 2
  • tubulin alpha-8 chain
  • tubulin, alpha 8

Background

Microtubules are cylindrical tubes of 20-25 nm in diameter. They are composed of protofilaments which are in turn composed of alpha- and beta-tubulin polymers. Each microtubule is polarized, at one end alpha-subunits are exposed (-) and at the other beta-subunits are exposed (+). Microtubules act as a scaffold to determine cell shape, and provide a backbone for cell organelles and vesicles to move on, a process that requires motor proteins. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (-) end. Microtubules also form the spindle fibers for separating chromosomes during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TUBA8
Entrez