| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | Flow |
| Clone | 8G7G3/1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | PE |
| Immunogen | Rat full length TTF-1 recombinant protein |
| Localization | Nuclear |
| Marker | Thyroid & Lung Epithelial Marker |
| Specificity | Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NKX2-1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for TTF1 / NKX2.1 Antibody (NBP2-34544PE)Discover more about diseases related to TTF1 / NKX2.1 Antibody (NBP2-34544PE).
| Pathways for TTF1 / NKX2.1 Antibody (NBP2-34544PE)View related products by pathway.
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TTF1 / NKX2.1 - An essential regulator of lung development with implications in cancer diagnostics Thyroid transcription factor 1 (TTF-1), also known as NKX2.1, is a conserved master regulatory transcription factor involved in the development of the lung, brain, and thyroid (1). In the lung TTF-1 positively regulates the expression of several lu... Read full blog post. |
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![Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).](http://images.novusbio.com/fullsize2/Thyroglobulin Antibody (2H11+6E1)-Immunohistochemistry-Paraffin-NBP2-34294-img0003.jpg)
![Western Blot: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - KRT20 monoclonal antibody (M01), clone 2G3-1C8 Analysis of KRT20 expression in A-431.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-(2G3-1C8)-Western-Blot-H00054474-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on U-2 OS cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-(2G3-1C8)-Immunocytochemistry-Immunofluorescence-H00054474-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (2G3-1C8) [H00054474-M01] - Analysis of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-(2G3-1C8)-Immunohistochemistry-Paraffin-H00054474-M01-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.](http://images.novusbio.com/fullsize2/Substance P Antibody-Immunocytochemistry-Immunofluorescence-NB300-187-img0003.jpg)
![Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).](http://images.novusbio.com/fullsize2/Substance P Antibody-Immunohistochemistry-NB300-187-img0002.jpg)
![Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in human cell line SiHa.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-Western-Blot-NBP1-88080-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-88080-img0003.jpg)
![Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/Cytokeratin 7 Antibody-Western Blot-NBP1-88080-img0009.jpg)
![Western Blot: Surfactant Protein B Antibody [NBP1-57977] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize2/Surfactant-Protein-B-Antibody-Western-Blot-NBP1-57977-img0003.jpg)
![Immunohistochemistry: Surfactant Protein B Antibody [NBP1-57977] - Human Lung Tissue Observed Staining: Cytoplasm and membrane of pneumocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec.](http://images.novusbio.com/fullsize2/Surfactant Protein B Antibody-Immunohistochemistry-NBP1-57977-img0004.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/PAX8-Antibody-Western-Blot-NBP1-32440-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32440-img0011.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Paraffin-embedded mouse liver. PAX8 antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0018.jpg)
![Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.](http://images.novusbio.com/fullsize2/Prosurfactant Protein C Antibody-Western Blot-NBP1-87201-img0005.jpg)
![Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human lung shows distinct cytoplasmic positivity in Type II pneumocytes.](http://images.novusbio.com/fullsize2/Prosurfactant-Protein-C-Antibody-Immunohistochemistry-Paraffin-NBP1-87201-img0001.jpg)
![Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.](http://images.novusbio.com/fullsize2/CKAP4-p63-Antibody-Western-Blot-NBP1-26642-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP1-26642] - FFPE section of human placenta. Antibody used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize2/CKAP4-p63-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26642-img0006.jpg)
![Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize2/CKAP4-p63-Antibody-Immunohistochemistry-Paraffin-NBP1-26642-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - (2ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize2/FOXE1 Antibody-Immunohistochemistry-Paraffin-NB100-1271-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - IHC analysis of paraffin embedded Human Testis using this antibody at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/FOXE1 Antibody-Immunohistochemistry-Paraffin-NB100-1271-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19788-img0005.jpg)
![Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Analysis in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-Immunohistochemistry-NBP1-19788-img0006.jpg)
![Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Western-Blot-NB100-355-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Immunocytochemistry-Immunofluorescence-NB100-355-img0006.jpg)
![Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).](http://images.novusbio.com/fullsize2/RPE65-Antibody-(401.8B11.3D9)-Immunohistochemistry-Frozen-NB100-355-img0007.jpg)
![Western Blot: UVRAG Antibody [NBP2-24482] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.](http://images.novusbio.com/fullsize2/UVRAG Antibody-Western Blot-NBP2-24482-img0001.jpg)
![Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Western-Blot-NB300-653-img0001.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain dilution 1:100](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0003.jpg)
![Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.](http://images.novusbio.com/fullsize2/Synaptophysin-Antibody-Immunohistochemistry-Paraffin-NB300-653-img0002.jpg)
![Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/DDT-Antibody-(1H3)-Western-Blot-NBP2-45677-img0008.jpg)
![Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/DDT-Antibody-(1H3)-Immunohistochemistry-NBP2-45677-img0007.jpg)
![Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.](http://images.novusbio.com/fullsize2/DDT Antibody (1H3)-Western Blot-NBP2-45677-img0009.jpg)
![SDS-Page: TTF1 / NKX2.1 Partial Recombinant Protein [H00007080-Q01] - 12.5%, Stained with Coomassie Blue.](http://images.novusbio.com/images2/TTF1---NKX2.1-Partial-Recombinant-Protein-SDS-Page-H00007080-Q01-img0001.jpg "SDS-Page: TTF1 / NKX2.1 Partial Recombinant Protein [H00007080-Q01] - 12.5%, Stained with Coomassie Blue.")
![Western Blot: TTF1 / NKX2.1 Overexpression Lysate (Denatured) [H00007080-T01] - Lane 1: NKX2-1 transfected lysate (40.92 KDa) Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/TTF1---NKX2.1-Overexpression-Lysate-(Denatured)-Western-Blot-H00007080-T01-img0002.jpg "Western Blot: TTF1 / NKX2.1 Overexpression Lysate (Denatured) [H00007080-T01] - Lane 1: NKX2-1 transfected lysate (40.92 KDa) Lane 2: Non-transfected lysate.")
![SDS-Page: TTF1 / NKX2.1 Overexpression Lysate (Denatured) [H00007080-T01] - NKX2-1 transfected lysate.](http://images.novusbio.com/images2/TTF1---NKX2.1-Overexpression-Lysate-(Denatured)-SDS-Page-H00007080-T01-img0001.jpg "SDS-Page: TTF1 / NKX2.1 Overexpression Lysate (Denatured) [H00007080-T01] - NKX2-1 transfected lysate.")
![Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.](http://images.novusbio.com/images2/RPE65-Antibody-(401.8B11.3D9)-Western-Blot-NB100-355-img0008.jpg "Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.")
![Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.](http://images.novusbio.com/images2/RPE65-Antibody-(401.8B11.3D9)-Immunocytochemistry-Immunofluorescence-NB100-355-img0006.jpg "Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [NB100-355] - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.")
![Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).](http://images.novusbio.com/images2/RPE65-Antibody-(401.8B11.3D9)-Immunohistochemistry-Frozen-NB100-355-img0007.jpg "Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).")