TSPAN8/TM4SF3 Overexpression Lysate (Denatured)

Images

 
TSPAN8 transfected lysate.
Lane 1: TSPAN8 transfected lysate (26.18 KDa) Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TSPAN8/TM4SF3 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-TSPAN8 full-length
Gene
TSPAN8

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
H00007103-T01 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for Array

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-TSPAN8 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TSPAN8/TM4SF3 Overexpression Lysate (Denatured)

  • CO-029
  • tetraspanin 8
  • TM4SF3
  • TM4SF3tspan-8
  • Transmembrane 4 superfamily member 3tetraspanin-8
  • TSPAN8
  • Tspan-8
  • Tumor-associated antigen CO-029

Background

Plasmid: pCMV-TSPAN8 Full length

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Applications: WB

Publications for TSPAN8/TM4SF3 Lysate (H00007103-T01) (0)

There are no publications for TSPAN8/TM4SF3 Lysate (H00007103-T01).
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Review for TSPAN8/TM4SF3 Lysate (H00007103-T01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00007103-T01:
Filter by Applications
WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot TSPAN8/TM4SF3 H00007103-T01
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reviewed by:
Ming Lu
WB Human 07/03/2014
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Summary

ApplicationWestern Blot
Sample TestedTetraspanin 8 293T cell transient overexpression lysate
SpeciesHuman
Lot07357705wuo
CommentsTM4SF3/TSPAN8 is not easy to work with in western blot due to it is a membrane protein with 4 trans-membrane domains. This overexpression lysate is a great control to make sure that your western actually works.

Blocking

Blocking Details2% BSA in PBS buffer.

Primary Anitbody

Dilution RatioTM4SF3 Antibody (1E5), 1:250 dilution 4C overnight

Secondary Antibody

Secondary Descriptiongoat anti mouse IgG HRP
Secondary Manufacturer Cat#Cellsignaling, Cat# 7076S
Secondary Concentration1:3000

Details

Detection NotesECL, exposure time 5 min

Comments

CommentsTM4SF3/TSPAN8 is not easy to work with in western blot due to it is a membrane protein with 4 trans-membrane domains. This overexpression lysate is a great control to make sure that your western actually works.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TSPAN8/TM4SF3 Lysate (H00007103-T01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

H00007103-T01

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Recent Reviews

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1
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Ming Lu
07/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol TSPAN8