TSLP R/CRLF2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG ...read more
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified ...read more
TSLP R was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) at 5 µg/mL overnight at 4 °C. Tissue ...read more
Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

TSLP R/CRLF2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSLP R
Val30-Phe232
Accession # Q9HC73
Specificity
Detects human TSLP R in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRLF2
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF981 in the following applications:

Publications
Read Publications using
AF981 in the following applications:

  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLP R/CRLF2 Antibody [Unconjugated]

  • CRL2
  • CRL2cytokine receptor CRL2 precusor
  • CRLF2
  • CRLF2Y
  • CRLM-2
  • Cytokine receptor-like 2
  • cytokine receptor-like factor 2
  • ILXR
  • IL-XR
  • P2RY8/CRLF2 fusion
  • PCOR1
  • Thymic stromal lymphopoietin protein receptor
  • thymic stromal lymphopoietin receptor
  • thymic stromal-derived lymphopoietin receptor
  • TSLP R
  • TSLP receptor
  • TSLPR

Background

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS‑like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLP R and IL-7 R alpha are coexpressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell‑attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).

  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
  5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

1398-TS/CF
Species: Hu
NBP2-67429
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF2168
Species: Hu, Mu
Applications: WB, Simple Western, ChIP
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
AF4984
Species: Hu
Applications: WB, Simple Western, ChIP, CyTOF-ready, ICC, ICFlow
MAB4260
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
AF284
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
DDX0700P-100
Species: Hu, Ca
Applications: B/N, Flow, IP
AF981
Species: Hu
Applications: WB, IHC, B/N, ICC/IF

Publications for TSLP R/CRLF2 Antibody (AF981)(3)

Reviews for TSLP R/CRLF2 Antibody (AF981) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF981:
Filter by Applications
WB
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20624642
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20624642

Details

Detection NotesSee PMID 20624642

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 20624642.
 
reviewed by:
Anonymous
Flow Human 01/12/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20624642
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20624642

Details

Detection NotesSee PMID 20624642

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 20624642

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TSLP R/CRLF2 Antibody (AF981) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for TSLP R/CRLF2 Antibody (AF981)

Discover related pathways, diseases and genes to TSLP R/CRLF2 Antibody (AF981). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TSLP R/CRLF2 Antibody (AF981)

Discover more about diseases related to TSLP R/CRLF2 Antibody (AF981).
 

Pathways for TSLP R/CRLF2 Antibody (AF981)

View related products by pathway.

PTMs for TSLP R/CRLF2 Antibody (AF981)

Learn more about PTMs related to TSLP R/CRLF2 Antibody (AF981).

Blogs on TSLP R/CRLF2

There are no specific blogs for TSLP R/CRLF2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

TSLP R/CRLF2
546-TR-050

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/12/2015
Application: WB
Species: Human

 
Anonymous
01/12/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CRLF2
Entrez
Uniprot