Recombinant Human TSLP Protein

Product Discontinued

Recombinant Human TSLP Protein Summary

• Description: A partial protein corresponding to TSLP.
• Preparation Method: Insect cell expressed amino acids Y29-Q159 of hTSLP (accession #NM_033035).
• Details of Functionality: This recombinant human TSLP has been tested for TARC induction by normal human peripheral blood cells with an observed ED50 of 10 ng/ml, which corresponds to a specific activity of approximately 1x10^5Units/mg.
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: TSLP
• Endotoxin Note: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

• Dilutions:
  • Immunoassay

Reactivity Notes

This protein was made to the human sequence.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile liquid; 20 mM NaH2PO4, 0.6M NaCl, pH 7.2. 0.22 um filtered.
• Preservative: 0.09% Sodium Azide

Notes

Purity: Greater than 95%, as determined by SDS-PAGE

Alternate Names for Recombinant Human TSLP Protein

• thymic stromal lymphopoietin
• TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. This hemopoietic cytokine was identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development. TSLP is expressed in thymus, spleen, kidney, lung and bone marrow. The bioactivity of TSLP overlaps with that of IL-7, playing a key role in B cell development, as well as stimulating the growth of thymocytes and T cells. Whereas IL-7 facilitates the development of B220+/IgM- pre-B cells, TSLP promotes the development B220+/IgM+ B cells in the mouse. In addition, TSLP stimulates monocytes to produce the T cell-attracting chemokines TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines (e.g. IL-4, IL-5, IL-13, and TNFalpha). TSLP binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Ralpha) and the TSLP-specific chain (TSLPR) to induce STAT3 and STAT5 phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant human TSLP is a 15.0 kDa protein consisting of 121 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSLP