Human TSLP ELISA Kit (Colorimetric)

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ELISA: TSLP ELISA Kit [DDXK-E-TSLP] - Each laboratory should establish its own standard curve. Example of standard curve using serial dilutions of TSLP (20ng/ml) prepared from supernatants of TSLP-transfected ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
human cell culture supernatants, plasma, serum, and various biological fluids.
Standard Curve Range
0.31 - 20ng/ml
Sensitivity
The minimum detectable dose of human TSLP was estimated at 1ng/ml

Order Details

Human TSLP ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.31 - 20ng/ml
Sensitivity
The minimum detectable dose of human TSLP was estimated at 1ng/ml
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TSLP

Applications/Dilutions

Application Notes
This is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Standard: Each vial contains 1ug/mL of recombinant TSLP produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended
  2. Capture Antibody: 0.5 mg/mL of mouse anti-TSLP monoclonal antibody (clone 209A2.03). Dilute in carbonate buffer pH9.6 to a working concentration of 2.5 ug /mL
  3. Detection Antibody: 0.5mg/mL of Biotin-conjugated anti-TSLP mouse monoclonal antibody (clone 210H1.04). Dilute to a working concentration of 3ug/mL in PBS-BSA1%-Tween 0.05%.

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for Human TSLP ELISA Kit (Colorimetric)

  • thymic stromal lymphopoietin
  • TSLP

Background

TSLP (thymic stromal lymphopoietin) is a hemopoietic protein of 159 aa (18 kDa), produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromallike cells. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is also detected in the thymus and the tissue cells of the bone marrow. TSLP affects the transition from pre-B cells in B. Alternative splicing of this gene results in two transcripts variants. TSLP is proposed to signal through a heterodimeric receptor complex composed of TSLP-receptor and IL7R chain. Expressed in many tissues, it prevents apoptosis and stimulates the growth of myeloid cells. (Reche PA et al, J Immunol., 2001, 167(1):336-43; Dorshkind. K, Nature. Immunol., 2000 (1):369-370). Monoclonal antibodies raised against human TSLP were obtained after mice immunization with TSLP-His-tagged transfected 293T cells, and were used to set up a quantitative immunoassay to determine TSLP concentration in biological samples

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol TSLP
Entrez