Troponin I Type 3 (cardiac) Antibody (4E5)

Images

 
Western Blot: Troponin I Type 3 (cardiac) Antibody (4E5) [NBP1-48026] Analysis of extracts (35ug) from 9 different cell lines by using anti-Troponin I Type 3 (cardiac) monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (4E5) [NBP1-48026] - Immunofluorescent staining of HeLa cells using anti-Troponin I Type 3 (cardiac) mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (4E5) [NBP1-48026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Troponin I Type 3 (cardiac).
Western Blot: Troponin I Type 3 (cardiac) Antibody (4E5) [NBP1-48026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Troponin I Type 3 (cardiac) (Right lane) cDNA for 48 hrs and ...read more
Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody (4E5) [NBP1-48026] - Immunofluorescent staining of A549 cells using anti-Troponin I Type 3 (cardiac) mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clone
4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Troponin I Type 3 (cardiac) Antibody (4E5) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 1-210 of human TNNI3
Specificity
This antibody is specific for Homo sapiens troponin I type 3 (cardiac) (TNNI3).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNI3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-5000
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Human, Primate, Canine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (4E5)

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • cTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1troponin I, cardiac
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for Troponin I Type 3 (cardiac) Antibody (NBP1-48026)

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Diseases for Troponin I Type 3 (cardiac) Antibody (NBP1-48026)

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Blogs on Troponin I Type 3 (cardiac).

Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI3
Entrez