Recombinant Human Troponin C (skeletal muscle) Protein

Images

 
SDS-Page: Troponin C (cardiac) Protein [NBC1-28768] - Troponin C (cardiac) 1-160aa, 18.1 kDa (160aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Troponin C (skeletal muscle) Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids1-160 of E.coli Troponin C (skeletal muscle)

Source: E.coli

Amino Acid Sequence: MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT PTKEELDAII EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF RIFDRNADGY IDPEELAEIF RASGEHVTDE EIESLMKDGD KNNDGRIDFD EFLKMMEGVQ

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNNC2
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 100mM NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Background

Troponin C, skeletal muscle, also known as TNNC2, is the central regulatory protein of striated muscle contraction, and modulates the Ca2+-activation characteristics of muscle fibers. Troponin has 3 subunits, Troponin I(Tn-1), Troponin T(Tn-T) and Troponin C(Tn-C). Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. Mutations in all components of this complex have been associated with skeletal muscle disease. Recombinant human Troponin C was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Troponin C (skeletal muscle) NBC1-28768

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Bioinformatics

Gene Symbol TNNC2