Tripeptidyl-Peptidase I/TPP1 Antibody

Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-36742] - (0.3ug/ml) Analysis of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tripeptidyl-Peptidase I/TPP1 Antibody Summary

• Description: Novus Biologicals Goat Tripeptidyl-Peptidase I/TPP1 Antibody (NBP1-36742) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Tripeptidyl-Peptidase I/TPP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TPSVIRKRYNLTSQD corresponding to internal region according to NP_000382.3.
• Epitope: internal region
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 65 kDa band observed in human placenta lysates (calculated MW of 61.2 kDa band according to NP_000382.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tripeptidyl-Peptidase I/TPP1 Antibody

• Cell growth-inhibiting gene 1 protein
• ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease)
• CLN2
• CLN2EC 3.4.14.9
• growth-inhibiting protein 1
• LINCL
• LPIC
• lysosomal pepstatin insensitive protease
• Lysosomal pepstatin-insensitive protease
• MGC21297
• TPP1
• TPP-1
• TPP-I
• Tripeptidyl aminopeptidase
• tripeptidyl peptidase I
• tripeptidyl-peptidase 1
• TripeptidylPeptidase I
• Tripeptidyl-Peptidase I

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tripeptidyl-Peptidase I/TPP1 Antibody (NBP1-36742)(1)

Publication using NBP1-36742

• Kousi M, Siintola E, Dvorakova L et al. Mutations in CLN7/MFSD8 are a common cause of variant late-infantile neuronal ceroid lipofuscinosis. Brain 2009-03-01 [PMID: 19201763]

Bioinformatics

• Gene Symbol: TPP1
• Entrez:
  • 1200(Human)
  • 12751(Mouse)
• Uniprot:
  • NP_000382.3(Human)