TRIM29 Antibody

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Dot Blot: TRIM29 Antibody [NBP1-77961] - This antibody shows reactivity by dot blot with acetylated and non-acetylated forms of the immunizing peptide. This antibody is predicted to recognize both acetylated (AcK116) ...read more

Product Details

Summary
Reactivity Hu, Bv, Eq, PmSpecies Glossary
Applications WB, DB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRIM29 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Specificity
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated and non-acetylated (at K116) ATDC protein.  A BLAST analysis was used to suggest cross-reactivity with AT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM29
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Dot Blot 1:100-1:2000
  • ELISA 1:100000
Application Notes
Anti-ATDC affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC should be optimized by the end user. Expect a band approximately ~66 kDa in size corresponding - ATDC by western blotting in the appropriate cell lysate or extract.
Control
TRIM29 Lysate (NBP2-65384)

Reactivity Notes

Reactive in human, horse, bovine, chimpanzee, macaque

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIM29 Antibody

  • Ataxia telangiectasia group D-associated protein
  • ATDC ataxia-telangiectasia group D-associated protein
  • ATDC
  • FLJ36085
  • tripartite motif containing 29
  • tripartite motif protein TRIM29
  • tripartite motif-containing 29
  • tripartite motif-containing protein 29

Background

Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM29
Uniprot