TRIM25 Antibody

Images

 
Western Blot: TRIM25 Antibody [NBP1-00114] - Detection of Human TRIM25 on HeLa whole cell lysate using NBP1-00114. TRIM25 was also immunoprecipitated by rabbit anti-TRIM25 antibodies NBP1-00113 and NBP1-00115.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00114
    • Availability
      Product Discontinued

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TRIM25 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human tripartite motif-containing 25 using the numbering given in entry NP_005073.2 (GeneID 7706).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM25
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TRIM25 Lysate (NBL1-17285)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM25 Antibody

  • EC 6.3.2.19
  • EC 6.3.2.n3
  • EFPZ147
  • Estrogen-responsive finger protein
  • RING finger protein 147
  • RNF147E3 ubiquitin/ISG15 ligase TRIM25
  • tripartite motif containing 25
  • tripartite motif-containing 25
  • Tripartite motif-containing protein 25
  • Ubiquitin/ISG15-conjugating enzyme TRIM25
  • zinc finger protein 147 (estrogen-responsive finger protein)
  • Zinc finger protein 147
  • zinc finger protein-147
  • ZNF147tripartite motif protein TRIM25

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TRIM25 Antibody (NBP1-00114)

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Bioinformatics

Gene Symbol TRIM25
Entrez
Uniprot