TRF-1 Antibody

Product Discontinued

TRF-1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Telomeric Repeat Binding Factor 1. The immunogen region is: Internal.
• Marker: Telomere Marker
• Specificity: Telomeric Repeat Binding Factor 1 (Ab-219) Antibody detects endogenous levels of total Telomeric Repeat Binding Factor 1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TERF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRF-1 Antibody

• NIMA-interacting protein 2
• PIN-2
• PIN2hTRF1-AS
• Telomeric protein Pin2/TRF1
• telomeric repeat binding factor (NIMA-interacting) 1
• telomeric repeat binding factor 1
• telomeric repeat binding protein 1
• telomeric repeat-binding factor 1
• TERF1
• TRBF1
• TRF1
• TRF-1
• TRF1FLJ41416
• TRFt-TRF1
• TTAGGG repeat-binding factor 1

Background

TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG. TRF1 and TRF2 are key components of vertebrate telomeres and bind to double-stranded telomeric DNA as homodimers. Dimerization involves the TRF homology (TRFH) domain, which also mediates interactions with other telomeric proteins. Despite a modest sequence identity, the 2 TRFH domains have the same entirely alpha-helical architecture, resembling a twisted horseshoe. The dimerization interfaces feature unique interactions that prevent heterodimerization. Mutational analysis of TRF1 corroborated the structural data and underscored the importance of the TRFH domain in dimerization, DNA binding, and telomere localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TERF1
• Uniprot:
  • P54274()