TREM-2 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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TREM-2 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-2b
Leu19-Pro168
Accession # Q99NH8
Specificity
Detects mouse TREM-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TREM-2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM-1 and rmTREM-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TREM2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1729 in the following applications:

Publications
Read Publications using
AF1729 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREM-2 Antibody

  • TREM2
  • TREM-2
  • Trem2a
  • Trem2b
  • Trem2c
  • TREM-2triggering receptor expressed on myeloid cells 2a
  • Triggering receptor expressed on monocytes 2
  • triggering receptor expressed on myeloid cells 2

Background

TREM-2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM-2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM‑2a and TREM‑2b. TREM-2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM‑2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM‑2 on immature DC with anti-TREM‑2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM‑2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM‑2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM‑2 deficient individuals (6).

  1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
  2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
  3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
  4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
  5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
  6. Cella, M. et al. (2003) J. Exp. Med. 198:645.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TREM-2 Antibody (AF1729)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC Frozen, WB.


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ICC
(1)
IHC
(5)
IHC Frozen
(1)
WB
(1)
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Mouse
(7)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF1729 Applications Species
PJ Cheng-Hath, EG Reed-Geagh, TR Jay, BT Casali, SM Bemiller, SS Puntambeka, VE von Saucke, RY Williams, JC Karlo, M Moutinho, G Xu, RM Ransohoff, BT Lamb, GE Landreth The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease Mol Neurodegener, 2018;13(1):29. 2018 [PMID: 29859094] (IHC,, Mouse) IHC Mouse
Y Zhao, X Li, T Huang, LL Jiang, Z Tan, M Zhang, IH Cheng, X Wang, G Bu, YW Zhang, Q Wang, H Xu Intracellular trafficking of TREM2 is regulated by presenilin 1 Exp. Mol. Med., 2017;49(12):e405. 2017 [PMID: 29611543] (WB,, Mouse) WB Mouse
KN Nam, A Mounier, CM Wolfe, NF Fitz, AY Carter, EL Castranio, HI Kamboh, VL Reeves, J Wang, X Han, J Schug, I Lefterov, R Koldamova Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice Sci Rep, 2017;7(1):4307. 2017 [PMID: 28655926] (IHC,, Mouse) IHC Mouse
R Saika, H Sakuma, D Noto, S Yamaguchi, T Yamamura, S Miyake MicroRNA-101a regulates microglial morphology and inflammation J Neuroinflammation, 2017;14(1):109. 2017 [PMID: 28558818] (ICC,, Mouse) ICC Mouse
EL Castranio, A Mounier, CM Wolfe, KN Nam, NF Fitz, F Letronne, J Schug, R Koldamova, I Lefterov Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury Neurobiol. Dis., 2017;0(0):. 2017 [PMID: 28502803] (IHC,, Mouse) IHC Mouse
Z Zhang, Y Takeda-Uch, T Foyez, S Ohtake-Nii, Narentuya, H Akatsu, K Nishitsuji, M Michikawa, T Wyss-Coray, K Kadomatsu, K Uchimura Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28320965] (IHC,, Mouse) IHC Mouse
Chertoff M, Shrivastava K, Gonzalez B, Acarin L, Gimenez-Llort L Differential modulation of TREM2 protein during postnatal brain development in mice. PLoS ONE, 2013;8(8):e72083. 2013 [PMID: 23977213] (IHC,, Mouse) IHC Mouse
Wohl SG, Schmeer CW, Friese T, Witte OW, Isenmann S In Situ Dividing and Phagocytosing Retinal Microglia Express Nestin, Vimentin, and NG2 In Vivo. PLoS ONE, 2011;6(8):e22408. 2011 [PMID: 21850226] (IHC Frozen,, Rat) IHC Frozen Rat

Review for TREM-2 Antibody (AF1729) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1729:
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(1)
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Images Ratings Applications Species Date Details
 TREM-2 AF1729
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reviewed by:
Anonymous
Mouse 03/20/2018
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Summary

Sample Tested Mouse brain
SpeciesMouse
Lotjpn0117071

Comments

Comments1:250 dilution, antigen retrieval for 15mins.

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Recent Reviews

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1
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Anonymous
03/20/2018
Application:
Species: Mouse

Bioinformatics

Gene Symbol TREM2
Entrez
Uniprot