TREM-1 Antibody (TREM-37)

Images

 
Flow Cytometry: TREM-1 Antibody (TREM-37) [NB100-77977] - Human peripheral blood granulocytes were stained with purified CD354 (clone TREM-37, filled histogram) or mouse IgG2b, kappa isotype control, followed by ...read more
Flow Cytometry: TREM-1 Antibody (TREM-37) [NB100-77977]

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-77977
    • Availability
      Product Discontinued

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TREM-1 Antibody (TREM-37) Summary

Immunogen
Recombinant human TREM-1
Specificity
TREM-1 (TREM-37)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TREM1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Application Notes
Each lot of this antibody is quality control tested by ELISA analysis and/or Western blotting. For ELISA capture applications, the suggested use of this reagent is 2-6 microgram/ml. To obtain a linear standard curve, serial dilutions of TREM-1 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 1 microgram/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
TREM-1 Lysate (NBL1-17264)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for TREM-1 Antibody (TREM-37)

  • CD354 antigen
  • CD354
  • TREM1
  • TREM-1
  • TREM-1Triggering receptor expressed on monocytes 1
  • triggering receptor expressed on myeloid cells 1
  • triggering-receptor TREM1

Background

TREM-1 is a 30 kD glycoprotein also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. It is a Type I membrane protein that contains an immunoglobulin-like V-type domain. Alternatively spliced protein variant may be secreted. TREM-1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM-1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM-1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM-1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock. This antibody has been shown to be useful for Western blotting, flow cytometric staining, and ELISA pairing with biotinylated TREM-26 antibody (Cat. No. 314904) as a detection antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TREM-1 Antibody (NB100-77977)

Discover related pathways, diseases and genes to TREM-1 Antibody (NB100-77977). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TREM-1 Antibody (NB100-77977)

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Research Areas for TREM-1 Antibody (NB100-77977)

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Blogs on TREM-1.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol TREM1
Entrez