TRAP220/MED1 Antibody

Images

 
Western Blot: TRAP220 Antibody [NB100-92386] - Western blot (WB) analysis of MED1 (P697) antibody in extracts from Jurkat cells.
Immunohistochemistry: TRAP220 Antibody [NB100-92386] - Immunohistochemistry (IHC) analyzes of MED1 (P697) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92386
    • Availability
      Product Discontinued

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TRAP220/MED1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 664-720 of Human TRAP220.
Specificity
MED1 (P697) detects endogenous levels of MED1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
MED1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500- 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of MED1 (P697) pAb in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of MED1 (P697) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAP220/MED1 Antibody

  • Activator-recruited cofactor 205 kDa component
  • ARC205
  • CRSP1TRIP2
  • CRSP200p53 regulatory protein RB18A
  • DRIP230
  • DRIP230PPAR-binding protein
  • MBD4
  • MED1
  • mediator complex subunit 1DRIP205
  • mediator of RNA polymerase II transcription subunit 1
  • PBPPPARGBP
  • Peroxisome proliferator-activated receptor-binding protein
  • PPAR binding protein
  • PPARBP
  • PPARBPMGC71488
  • PPARG binding protein
  • PPARGBP
  • RB18ATR-interacting protein 2
  • Thyroid hormone receptor-associated protein complex 220 kDa component
  • thyroid hormone receptor-associated protein complex component TRAP220
  • thyroid receptor interacting protein 2
  • Thyroid receptor-interacting protein 2
  • TRAP220
  • TRAP220TRIP-2
  • TRIP2
  • vitamin D receptor-interacting protein 230 kD
  • Vitamin D receptor-interacting protein complex component DRIP205

Background

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors. These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation. The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, that are characterized by the presence of a nuclear receptor recognition motif which mediates the liganddependent binding of TRAP proteins to the nuclear receptors. TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta. TRAP95, TRAP150 and TRAP230 facilitate TR induced transcription by associating with an additional transcriptional coactivating complex SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MED1
Entrez