Recombinant Human TRAP1 Protein

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SDS-Page: Recombinant Human TRAP1 Protein [NBP1-99222]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
13.5 mg/ml

Order Details

Recombinant Human TRAP1 Protein Summary

Description
Recombinant protein containing 702 amino acids for Human TRAP1

Source:E. coli.
Amino Acid Sequence:(Q12931)

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TRAP1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Application Notes
Specific activity: Kd = 1.95 nM. Assay conditions: 20 mM HEPES, pH 7.5, 50 mM KCl, 5 mM MgCl2, 20 mM sodium molybdate, 2 mM DTT, 0.01% NP40, 100 ug/ml BSA, 50 nM TRAP1, geldanamycin-FITC (titration range 0.016-16 nM) incubated at 4oC for 24 hrs. Fluorescence polarization measured at RT (Ex/Em= 485/520 nm)
Theoretical MW
76.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Concentration
13.5 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human TRAP1 Protein

  • HSP 75
  • HSP75TNFR-associated protein 1
  • HSP90Lheat shock protein 75 kDa, mitochondrial
  • TNF receptor-associated protein 1
  • TRAP-1
  • tumor necrosis factor type 1 receptor associated protein
  • Tumor necrosis factor type 1 receptor-associated protein

Background

Human TRAP1 belongs to the mitochondrial heat shock protein family. These molecular chaperones are highly conserved and play an important role in signal transduction, protein folding and degradation. Recombinant human TRAP1 has a C-terminal FLAG tag and has 702 amino acid residues. It has been identified to be protective for cell survival, and has been suggested as a target for anti-cancer therapeutics. Also can be useful for studies including enzyme kinetics, activator screening and selectivity profiling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol TRAP1