TRAP1 Antibody (1H8)

Images

 
Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1.
Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human Kidney tissue using anti-TRAP1 mouse monoclonal antibody.
Flow Cytometry: TRAP1 Antibody (1H8) [NBP2-01707] - Analysis of Jurkat cells, using anti-TRAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Rt, Ca, MkSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TRAP1 Antibody (1H8) Summary

Immunogen
Full length human recombinant protein of human TRAP1(NP_057376) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TRAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRAP1 Antibody (1H8)

  • HSP 75
  • HSP75TNFR-associated protein 1
  • HSP90Lheat shock protein 75 kDa, mitochondrial
  • TNF receptor-associated protein 1
  • TRAP-1
  • tumor necrosis factor type 1 receptor associated protein
  • Tumor necrosis factor type 1 receptor-associated protein

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRAP1
Entrez