TRAP alpha Antibody (4C7)

Images

 
Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP alpha.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.
Flow Cytometry: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAP alpha antibody, and then ...read more
Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP alpha monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Immunofluorescent staining of HeLa cells using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human pancreas tissue using anti-TRAP alpha mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, Mk, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
4C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TRAP alpha Antibody (4C7) Summary

Immunogen
Full length human recombinant protein of human SSR1(NP_003135) produced in HEK293 cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SSR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01026 in the following applications:

Publications
Read Publication using
NBP2-01026 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRAP alpha Antibody (4C7)

  • DKFZp781N23103
  • FLJ14232
  • FLJ22100
  • FLJ23034
  • FLJ78242
  • FLJ93042
  • signal sequence receptor, alpha
  • SSR alpha subunit
  • SSR-alpha
  • translocon-associated protein alpha subunit
  • translocon-associated protein subunit alpha
  • TRAP alpha
  • TRAP-alpha
  • TRAPASignal sequence receptor subunit alpha

Background

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAP alpha Antibody (NBP2-01026)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for TRAP alpha Antibody (NBP2-01026) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-01026:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TRAP alpha NBP2-01026
Enlarge
reviewed by:
Marie-estelle LOSFELD
WB Human 02/27/2014
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Summary

ApplicationWestern Blot
Sample Testedhuman dermal fibroblasts
SpeciesHuman
LotA01

Blocking

Blocking Details5% milk in TBS-tween

Primary Anitbody

Dilution Ratio1/500 incubation 3h RT in 5% milk in TBS-tween

Secondary Antibody

Secondary Descriptionanti-Mouse IgG HRP from amersham
Secondary Concentration1/5000

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRAP alpha Antibody (NBP2-01026) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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1
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Marie-estelle LOSFELD
02/27/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SSR1
Entrez