Transgelin/TAGLN/SM22 alpha Antibody (8C8)

Images

 
Western Blot: SM22 alpha Antibody (8C8) [NBP2-00894] Analysis of extracts (35ug) from 9 different cell lines by using anti-SM22 alpha monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded human breast cancer tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope ...read more
Western Blot: SM22 alpha Antibody (8C8) [NBP2-00894] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SM22 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human breast tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human endometrium tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SM22 alpha Antibody (8C8) [NBP2-00894] - Staining of paraffin-embedded Human prostate tissue using anti-SM22 alpha mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
8C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Transgelin/TAGLN/SM22 alpha Antibody (8C8) Summary

Immunogen
Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150

Reactivity Notes

Human, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody (8C8)

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TAGLN
Entrez