Mouse TRANCE/TNFSF11/RANK L ELISA Kit (Colorimetric)

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ELISA: TRANCE/TNFSF11/RANK L ELISA Kit [Biotin] [KA1817] - These standard curves are for demonstration only. A standard curve must be run with each assay.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum
Sensitivity
4 pg/mL

Order Details

Mouse TRANCE/TNFSF11/RANK L ELISA Kit (Colorimetric) Summary

Description
Tnfsf11 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Tnfsf11.
Sensitivity
4 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 12%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFSF11

Applications/Dilutions

Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
  2. Trance Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse Trance.
  3. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
  4. Standards (Item C): 2 vials of recombinant mouse Trance.
  5. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  6. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For standard/sample (cell culture medium) diluent.
  7. Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
  8. Detection Antibody Trance (Item F): 2 vial of biotinylated anti-mouse Trance (each vial is enough to assay half microplate).
  9. HRP-Streptavidin Concentrate (Item G): 8 µl 10,000x concentrated HRP-conjugated streptavidin.

Notes

Quality control test: Standard Curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse TRANCE/TNFSF11/RANK L ELISA Kit (Colorimetric)

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on TRANCE/TNFSF11/RANK L.

The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Bioinformatics

Gene Symbol TNFSF11
Entrez