TRAILR1/TNFRSF10A Antibody (001) [Alexa Fluor® 647] Summary
| Additional Information |
Recombinant Monoclonal Antibody |
| Immunogen |
This antibody was obtained from a rabbit immunized with purified, recombinant Human TRAILR1/TNFRSF10A (NP_003835.2; Met1-Asn239). |
| Isotype |
IgG |
| Clonality |
Monoclonal |
| Host |
Rabbit |
| Gene |
TNFRSF10A |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- ELISA
- Sandwich ELISA Detection
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for TRAILR1/TNFRSF10A Antibody (001) [Alexa Fluor® 647]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for TRAILR1/TNFRSF10A Antibody (NBP2-89475AF647) (0)
Secondary Antibodies
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Isotype Controls
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Other Available Formats
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Diseases for TRAILR1/TNFRSF10A Antibody (NBP2-89475AF647)
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Blogs on TRAILR1/TNFRSF10A
![TRAIL/TNFSF10 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "TRAIL/TNFSF10 [Unconjugated]")
![Western Blot: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/TRAIL-R2-TNFRSF10B-Antibody-Western-Blot-NB100-56618-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody [NB100-56618] - TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer.](http://images.novusbio.com/images2/TRAIL-R2-TNFRSF10B-Antibody-Immunohistochemistry-Paraffin-NB100-56618-img0007.jpg)
![Immunohistochemistry: TRAILR2/TNFRSF10B Antibody [NB100-56618] - Pharmacodynamic assays for ONC201. IHC analyses of CHOP, TRAILR2/TNFRSF10B, and double-stranded DNA breaks (TUNEL) in baseline and ONC201-treated biopsies for an endometrial cancer patient. The biopsy was done 9.8 months after starting ONC201 treatment (7 days after the most recent ONC201 dose). Image collected and cropped by CiteAb from the following publication (https://jitc.biomedcentral.com/articles/10.1186/s40425-019-0599-8) licensed under a CC-BY licence.](http://images.novusbio.com/images2/TRAILR2-TNFRSF10B-Antibody-Immunohistochemistry-NB100-56618-img0008.jpg)
![Immunohistochemistry: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Reduced pancreatic Atg7 level increased the expression of Caspase-8 in 12-week-old Atg7deltapan mice. Caspase-8 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-8 (green) ( 1/1000, scale bar=50 um). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-8-Antibody---(active-cleaved)-Immunohistochemistry-NB100-56116-img0009.jpg)
![Western Blot: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Analysis of active/cleaved Caspase 8 in NRK whole cell lysate using anti-active/cleaved Caspase 8 antibody. Image from verified customer review.](http://images.novusbio.com/images2/Caspase-8-Antibody---(active-cleaved)-Western-Blot-NB100-56116-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).](http://images.novusbio.com/images2/Caspase-8-Antibody---(active-cleaved)-Immunohistochemistry-Paraffin-NB100-56116-img0004.jpg)
![Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12. Analysis of CFLAR expression in K-562.](http://images.novusbio.com/images2/FLIP-Antibody-(2F12)-Western-Blot-H00008837-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/FLIP-Antibody-(2F12)-Immunocytochemistry-Immunofluorescence-H00008837-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/FLIP-Antibody-(2F12)-Immunohistochemistry-Paraffin-H00008837-M01-img0009.jpg)
![Western Blot: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/images2/HLA-DRB1-Antibody-(SPM288)-Western-Blot-NBP2-45312-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Formalin-fixed, paraffin-embedded human Tonsil stained with HLA DRB1 Antibody (SPM288).](http://images.novusbio.com/images2/HLA-DRB1-Antibody-(SPM288)-Immunohistochemistry-Paraffin-NBP2-45312-img0004.jpg)
![Flow Cytometry: HLA DRB1 Antibody (SPM288) [NBP2-45312] - Flow Cytometric Analysis of human Raji cells using HLA DRB1 Antibody (SPM288).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA-DRB1-Antibody-(SPM288)-Flow-Cytometry-NBP2-45312-img0002.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0042.jpg)
![Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/images2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Simple-Western-NB100-56708-img0033.jpg)
![Western Blot: DR1 Antibody [NBP2-47480] - Analysis in control (vector only transfected HEK293T lysate) and DR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/DR1-Antibody-Western-Blot-NBP2-47480-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DR1 Antibody [NBP2-47480] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.](http://images.novusbio.com/images2/DR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47480-img0004.jpg)
![Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-47480] - Staining of human cerebral cortex.](http://images.novusbio.com/images2/DR1-Antibody-Immunohistochemistry-Paraffin-NBP2-47480-img0009.jpg)
![Western Blot: HLA DRB3 Antibody [NBP3-03661] - Analysis of extracts of Raji cells, using HLA DRB3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/images2/HLA DRB3 Antibody-Western Blot-NBP3-03661-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DRB3 Antibody [NBP3-03661] - Paraffin-embedded human appendix using HLA DRB3 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/HLA-DRB3-Antibody-Immunohistochemistry-Paraffin-NBP3-03661-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DRB3 Antibody [NBP3-03661] - Paraffin-embedded tonsil using HLA DRB3 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/images2/HLA-DRB3-Antibody-Immunohistochemistry-Paraffin-NBP3-03661-img0001.jpg)
![Western Blot: HLA DRB4 Antibody (4C8) [H00003126-M01] - HLA-DRB4 monoclonal antibody (M01), clone 4C8. Analysis of HLA-DRB4 expression in human kidney.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Western-Blot-H00003126-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DRB4 Antibody (4C8) [H00003126-M01] - Analysis of monoclonal antibody to HLA-DRB4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Immunohistochemistry-Paraffin-H00003126-M01-img0004.jpg)
![Sandwich ELISA: HLA DRB4 Antibody (4C8) [H00003126-M01] - Detection limit for recombinant GST tagged HLA-DRB4 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/HLA-DRB4-Antibody-(4C8)-Sandwich-ELISA-H00003126-M01-img0005.jpg)
![Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).](http://images.novusbio.com/images2/Bcl-xL-Antibody-Western-Blot-NB100-56104-img0007.jpg)
![Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.](http://images.novusbio.com/images2/Bcl-xL-Antibody-Western-Blot-NB100-56104-img0008.jpg)
![Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-xL expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-xL expression.](http://images.novusbio.com/images2/Bcl-Xl-Antibody-Immunohistochemistry-Paraffin-NB100-56104-img0006.jpg)
![Western Blot: HLA DRB5 Antibody [NBP2-14093] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/images2/HLA DRB5 Antibody-Western Blot-NBP2-14093-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DRB5 Antibody [NBP2-14093] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/images2/HLA-DRB5-Antibody-Immunohistochemistry-Paraffin-NBP2-14093-img0001.jpg)
![Western Blot: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis using HLA-B mAb against HEK293 (1) and HLA-B (AA: 241-362)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Western-Blot-NBP2-61871-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of Hela cells using HLA-B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Immunocytochemistry-Immunofluorescence-NBP2-61871-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA B Antibody (2G7B10) [NBP2-61871] - Analysis of bladder cancer tissues using HLA-B mouse mAb with DAB staining.](http://images.novusbio.com/images2/HLA-B-Antibody-(2G7B10)-Immunohistochemistry-NBP2-61871-img0006.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S).. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0014.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0013.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.](http://images.novusbio.com/images2/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56118-img0006.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0007.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.](http://images.novusbio.com/images2/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56098-img0011.jpg)