Mouse myeloma cell line NS0-derived recombinant human TRAIL (R&D Systems, Catalog # 375-TL) Thr95-Gly281 Accession # P50591
Detects human TRAIL in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) TRAIL, rhFas Ligand, rhOPG, rhTNF-alpha, and rhTRANCE is observed.
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tumor necrosis factor (ligand) superfamily, member 10
tumor necrosis factor ligand superfamily member 10
TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1-3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).
Golstein, P. (1997) Current Biology 7:R750.
Wiley, S.R. et al. (1995) Immunity 3:673.
Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
Emery, J. et al. (1998) J. Biol. Chem. 273:14363.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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